msc chondrogenic differentiation medium (PromoCell)

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PromoCell msc chondrogenic differentiation medium

Analysis of surface markers and multipotency of stromal cells in biosheets (A) Representative flow cytometry profiles of cells isolated from 2w-biosheets and identification of a population of quadruple-positive cells (P7). (B) The ratio of quadruple-positive cells (P7) to stromal cells (P5) in the biosheets and fascia. (C-E) Representative images of alkaline phosphatase (ALP) staining and Alizarin red staining (C), oil red staining (D), Toluidine blue (TB) staining and Safranine O (SO) staining (E) of stromal cells isolated from biosheets and subjected to osteogenic, adipogenic and <t>chondrogenic</t> differentiation. (F-H) Expression levels of osteogenic (F), adipogenic (G), and chondrogenic (H) marker genes. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Msc Chondrogenic Differentiation Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/msc+chondrogenic+differentiation+medium/Mesenchymal+Stem+Cell+Chondrogenic+Differentiation+Medium/pmc12969807-307-15-20
Average 95 stars, based on 75 article reviews

msc chondrogenic differentiation medium - by Bioz Stars, 2026-09

95/100 stars

Images

1) Product Images from "Hepatocyte growth factor in biosheets promotes autonomous regeneration of cutaneous tissue after transplantation onto a full-thickness skin defect"

Article Title: Hepatocyte growth factor in biosheets promotes autonomous regeneration of cutaneous tissue after transplantation onto a full-thickness skin defect

Journal: Materials Today Bio

doi: 10.1016/j.mtbio.2026.102969

Figure Legend Snippet: Analysis of surface markers and multipotency of stromal cells in biosheets (A) Representative flow cytometry profiles of cells isolated from 2w-biosheets and identification of a population of quadruple-positive cells (P7). (B) The ratio of quadruple-positive cells (P7) to stromal cells (P5) in the biosheets and fascia. (C-E) Representative images of alkaline phosphatase (ALP) staining and Alizarin red staining (C), oil red staining (D), Toluidine blue (TB) staining and Safranine O (SO) staining (E) of stromal cells isolated from biosheets and subjected to osteogenic, adipogenic and chondrogenic differentiation. (F-H) Expression levels of osteogenic (F), adipogenic (G), and chondrogenic (H) marker genes. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Techniques Used: Flow Cytometry, Isolation, Staining, Expressing, Marker

Staining:

Article Title: Exosomes derived from mesenchymal stem cells primed with disease-condition-serum improved therapeutic efficacy in a mouse rheumatoid arthritis model via enhanced TGF-β1 production.
Article Snippet: Adipogenesis and osteogenesis were confirmed by staining with Oil Red O (Mesenchymal Adipogenesis Kit; Millipore SCR020) and Alizarin Red S (A5533; Sigma-Aldrich), respectively. .. For chondrogenesis, 1 × 106 iMSCs were centrifuged in a 15 mL tube to form a pellet, differentiated using cartilage differentiation medium for up to 3 weeks, with fresh media changed every third day (MSC Chondrogenic Differentiation Medium, Promocell, Heidelberg, Germany), fixed in formalin, paraffin-sectioned, and stained with toluidine blue to confirm chondrogenesis. .. For exosome production, iMSCs were seeded in DMEM supplemented with 2 mmol/mL glutamine, 100 μg/ mL penicillin/streptomycin, and 10% fetal bovine serum (FBS) at a density of 107 per 175 T flask (about 6 × 104 cells/cm2) for 24 h, and then incubated in DMEM supplemented with 2 mmol/mL glutamine, 100 μg/mL penicillin/streptomycin, and 10% Exosome-depleted FBS (A2720801, Gibco, Grand Island, NY, USA) for 48 h (MSC-FBS).

Article Title: Exosomes derived from mesenchymal stem cells primed with disease-condition-serum improved therapeutic efficacy in a mouse rheumatoid arthritis model via enhanced TGF-β1 production
Article Snippet: Adipogenesis and osteogenesis were confirmed by staining with Oil Red O (Mesenchymal Adipogenesis Kit; Millipore SCR020) and Alizarin Red S (A5533; Sigma-Aldrich), respectively. .. For chondrogenesis, 1 × 10 6 iMSCs were centrifuged in a 15 mL tube to form a pellet, differentiated using cartilage differentiation medium for up to 3 weeks, with fresh media changed every third day (MSC Chondrogenic Differentiation Medium, Promocell, Heidelberg, Germany), fixed in formalin, paraffin-sectioned, and stained with toluidine blue to confirm chondrogenesis. .. For exosome production, iMSCs were seeded in DMEM supplemented with 2 mmol/mL glutamine, 100 μg/mL penicillin/streptomycin, and 10% fetal bovine serum (FBS) at a density of 10 7 per 175 T flask (about 6 × 10 4 cells/cm 2 ) for 24 h, and then incubated in DMEM supplemented with 2 mmol/mL glutamine, 100 μg/mL penicillin/streptomycin, and 10% Exosome-depleted FBS (A2720801, Gibco, Grand Island, NY, USA) for 48 h (MSC-FBS).

Cell Characterization:

Article Title: Differences in the stemness characteristics and molecular markers of distinct human oral tissue neural crest‐derived multilineage cells
Article Snippet: For adipogenic differentiation, the cells were cultured in adipogenic differentiation medium, (αMEM supplemented with 10% FBS and 500 mM 3‐isobutyl‐1‐methylxanthine [Sigma‐Aldrich, St. Louis, MO], 1 μM dexamethasone [Sigma‐Aldrich], 0.01 mg/ml insulin [Sigma‐Aldrich], and 0.2 mM indomethacin [Sigma‐Aldrich]). .. For chondrogenic differentiation, enzymatically dissociated cells (2.0 × 10 cells/tube) were maintained in MSC chondrogenic differentiation medium (ready‐to‐use; PromoCell) using a pellet culture., , For myogenic differentiation, spheres were prepared as described above and then cultured in high‐glucose DMEM supplemented with 10% FBS and 10 ng/ml TGF‐β1 (PeproTech)., , For neural differentiation, cells were cultured in MSC neurogenic differentiation medium (ready‐to‐use; PromoCell). ..

Cell Culture:

Article Title: Cycloastragenol as an Exogenous Enhancer of Chondrogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells. A Morphological Study
Article Snippet: .. After 24 h, the pellets were cultured in different conditions in triplicate: (1) chondrogenic medium (CM), MSC chondrogenic differentiation medium, (PromoCell, Heidelberg, Germany); (2) CM supplemented with CAG (0.1 μM), for 7, 14, 21, and 28 days. ..

Incubation:

Article Title: Electrochemiluminescence imaging of respiratory activity of cellular spheroids using sequential potential steps.
Article Snippet: The respiratory activity of cultured cells can be electrochemically monitored using scanning electrochemical microscopy (SECM) with high spatial resolution.. However, in SECM, the electrode takes a long time to scan, limiting simultaneous measurements with large biological samples such as cell spheroids.. Therefore, for rapid electrochemical imaging, a novel strategy is needed.

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